Regulatory

Part:BBa_K284008:Design

Designed by: Fabiana de Melo Duarte, Leonardo Minete, Maria Carolina Grassi and Lucas P. Parizzi   Group: iGEM09_UNICAMP-Brazil   (2009-08-06)

tra operon promoter (Py)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mechanism for controlling transcription from Py remains undefined. The Py region contains significant intrinsic curvature, which could interfere in transcription regulation.

Source

F-plasmid of Escherichia coli.

References

Will,W.R. and Frost, L.S. 2006. "Characterization of the Opposing Roles of H-NS and TraJ in Transcriptional Regulation of the F-Plasmid tra Operon" J. Bacteriol. vol 188(2): 507-514.

Will,W.R. and Frost, L.S. 2006. "Hfq is a regulator of F-Plasmid TraJ and TraM synthesis in Escherichia coli" J. Bacteriol. vol 188(1): 124-131