Regulatory

Part:BBa_K284002:Design

Designed by: Raissa Estrela Curado, Tais Sineiro Herig and Gleidson Silva Teixeira   Group: iGEM09_UNICAMP-Brazil   (2009-09-26)

JEN1 Promoter from Kluyveromyces lactis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes regulated by this promoter can be induced by lactate even in the presence of glucose.

Source

This part was amplified by PCR from Kluyverimyces lactis genomic DNA.

References

Queiros O, Pereira L, Paiva S, Moradas-Ferreira P and Casal M. 2007. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Curr Genet 51, 161-169.