Reporter
Part:BBa_K2835007:Design
Designed by: Emma Larsson, Hanna Norbäck Group: iGEM18_Stockholm (2018-09-29)
Constitutive reporter: strong promoter + RBS + mRFP1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Assembly: Strong constitutive promoter + strong RBS + mRFP1
Source
The RFP gene is originally derived from Discosoma striata (coral). The mRFP1 gene is originally derived from a monomeric derived version of RFP, GenBank: AF506027.1