Coding

Part:BBa_K2835005:Design

Designed by: Emma Larsson   Group: iGEM18_Stockholm   (2018-09-29)


His-tagged fungal laccase with start codon (ATG)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
    Illegal BglII site found at 1243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Start codon (ATG) added to this part, in order to be able to express it in a standard iGEM plasmid in for example E.coli.

6xHis-tag added at the N-terminal.

Source

Laccase gene (lcc2) from Trametes versicolor.

References