Protein_Domain

Part:BBa_K2835004:Design

Designed by: Emma Larsson   Group: iGEM18_Stockholm   (2018-09-15)


His-tagged laccase, mutated to increase the specificity for SMX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains mutated residues at points 162, 332 and 337, from Phenylalanine to Isoleucine.

Codon optimized for Pichia pastoris.

Source

Laccase gene (lcc2) originally from Trametes versicolor.

References