Composite
PspA

Part:BBa_K2832007:Design

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


PspF Inducible mRFP1 Generator ( PspF ---> PspAp ---> mRFP1 )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T--FSU--BBa_K2832007.png

Plasmid map of BBa_K2832007. PspA promoter, potentially sound sensitive via the pspF pathway.

Source

Source...

References