Composite
PspA

Part:BBa_K2832007

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


PspF Inducible mRFP1 Generator ( PspF ---> PspAp ---> mRFP1 )

Sound could disrupt the plasma membrane and deplete the proton motive force. The proton motive force could also be disrupted by the opening of mechanical sensitive channels, further description of msc's in zntA system information. psp system activates to restore membrane integrity and proton motive force. E Coli relies on a high concentration of protons outside the cell to work. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates RFP expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None