Composite
BamE

Part:BBa_K2832005

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


RpoE Inducible mRFP1 Generator (RpoE --> bamEp --> mRFP1)

General stress response sigma factor. Responds to stress on membrane and periplasmic proteins. Binding of unassembled outer membrane proteins to the protease DegS stimulate its cleavage of the regulatory protein RseA, which tethers sigma E to the inner membrane. bamE is a member of the sigma E regulon. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates the expression of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 769
    Illegal AgeI site found at 881
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None