Regulatory

Part:BBa_K2832004:Design

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


PzntA - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PzntA_Design_Diagram.png PzntA Design Diagram. The zntA promoter sequence binds ZntR when activated by zinc. ZntR is a zinc-dependent promoter and detects increasing zinc concentration, possible through the opening of a mechanosensitive channel. mRFP1 signal indicates successful activation of the pathway.

PzntA_plasmid_map.png

FSU Promoter BBa_K2832004 inside plasmid pSB1C3.

Source

E. coli K12 genome.

References