Regulatory
Part:BBa_K2832004
Designed by: Michael Taylor and Arianna Sigalos Group: iGEM18_FSU (2018-09-09)
PzntA - Inducible Promoter
The zntAp promoter upregulates the zntA gene for the ATPase zinc exporter, ZntA. To decrease high intracellular concentrations of zinc, the zntA gene expresses ZntA upon activation by the cytosolic zinc-bound transcription factor, ZntR. Zinc ions can enter the cell through mechanosensitive channels (Mscs) that are opened by a stress on the membrane, and can then bind to ZntR. The zntAp promoter is therefore indirectly activated by stress, in which four zinc ions must enter the cell through the Msc to bind and activate ZntR. Our binding sites for zinc-bound ZntR include -35 and -10 b.p. upstream of the zntAp promoter for mRFP upregulation. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 13
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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