Regulatory

Part:BBa_K2832000:Design

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


PbamE - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PbamE_design_diagram.png PbamE Design Diagram. The bamE promoter sequence binds Sigma Factor E (RpoE) when freed by the rpoE pathway. The pathway detects membrane damage via misfolded outer membrane proteins. mRFP1 signal indicates successful activation of the pathway.

PbamE_plasmid_map.png FSU Promoter BBa_K2832000 inside plasmid pSB1C3.

Source

E. coli K12 genome.

References