Regulatory
Part:BBa_K2832000:Design
Designed by: Michael Taylor and Arianna Sigalos Group: iGEM18_FSU (2018-09-09)
PbamE - Inducible Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 13
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PbamE Design Diagram. The bamE promoter sequence binds Sigma Factor E (RpoE) when freed by the rpoE pathway. The pathway detects membrane damage via misfolded outer membrane proteins. mRFP1 signal indicates successful activation of the pathway.
FSU Promoter BBa_K2832000 inside plasmid pSB1C3.
Source
E. coli K12 genome.