Composite

Part:BBa_K2824009:Design

Designed by: Jiang Qiaochu   Group: iGEM18_NEU_China_B   (2018-10-06)


LsrA promoter-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 897


Design Notes

In fact, the complete replication of the six genes that began with LsrA, that is, from LSRA to LSRG, is also a feasible method of construction. Only the single promoter has been able to recognize the signal molecule AI-2, so this construction is relatively simple and convenient.


Source

The promoter of LsrA is from the genome of Escherichia coli K12. GFP gene was a commercial components which is a green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP .

References