Composite

Part:BBa_K2824007:Design

Designed by: Jiang Qiaochu   Group: iGEM18_NEU_China_B   (2018-10-05)


lldPRD operon promoter-Luxs-Lldr


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 603
    Illegal AgeI site found at 1442
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 335


Design Notes

Note the need for mutations in the Luxs gene sequence. In addition, the distance between O _ 1 and O _ 2 of LLDPRD can be adjusted appropriately.


Source

All the genes are extracted from Escherichia coli K12 genome.

References