Composite

Part:BBa_K2824005:Design

Designed by: Jiang Qiaochu   Group: iGEM18_NEU_China_B   (2018-10-05)


lldPRD operon promoter-Luxs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 335


Design Notes

The Luxs gene needs to be mutated to fit the PSB1C3 plasmid because it contains the restriction site of ECOR I.


Source

Both of the genes are from Escherichia coli K12.

References