Coding

Part:BBa_K2824001:Design

Designed by: Jiang Qiaochu   Group: iGEM18_NEU_China_B   (2018-10-05)


Luxs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of Luxs gene contains the restriction sequence of ECOR I, which needs to be mutated before it can be constructed, expressed and functioned normally.


Source

The Luxs gene is from the Escherichia coli. K-12 genome.

References