Coding

Part:BBa_K2817004:Design

Designed by: Zhaoyu Liu   Group: iGEM18_NEU_China_A   (2018-09-25)


Myrosinase (horseradish)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 139
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 368
    Illegal BsaI site found at 1397


Design Notes

Combined with a vegetable diet, the sulforaphane produced by myrosinase can both alleviate inflammation in the intestine and prevent colorectal cancer induced by chronic inflammation.


Source

It comes from cruciferous plant. We did a codon optimization for K12 strain and gave it to the company to synthesize it.

Organism: Armoracia rusticana

References