Part:BBa_K2814023:Design

Second node_BxbI-Xis_Recombinase based IFFL

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 153
  Illegal NgoMIV site found at 1045
  Illegal AgeI site found at 921
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 117
  Illegal BsaI.rc site found at 1025
  

Design Notes

The orientation of attB and attP sites was maintained carefully

Source

We designed this part after going through the scientific literature.