Composite
Part:BBa_K2814016:Design
Designed by: Saksham Sharma Group: iGEM18_IIT_Delhi (2018-10-10)
Second Node_TP901_Recombinase based IFFL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 337
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 611
Illegal XhoI site found at 698 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1445
Design Notes
The orientation of recombinase sites was to be maintained throughout the design
Source
We designed this part after going through the scientific literature.