Coding

Part:BBa_K2804009

Designed by: Eduardo Alejandro Moncayo Palacio   Group: iGEM18_Ecuador   (2018-10-12)


CcpAx: Cellulose-complementing protein


CcpAx encodes a protein named cellulose-complementing protein which is a critical part of the Bacterial Cellulose synthesis terminal complex.

This gene is member of the cellulose synthase complex which in Gluconacetobacter xylinus, is located upstream of the bscABCD operon. CcpAx is co-localized with BcsD longitudinally on the extracellular side of the cell membrane and may facilitate protein-protein interactions for the spatial assembly of BC synthesis complexes. Disruption of ccpAx results in a significant reduction in the levels of BcsB and BcsC which are proteins members of the bacterial cellulose synthesis complex indicating CcpAx also plays a regulatory role in BC biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 13
    Illegal XhoI site found at 666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None