Composite

Part:BBa_K2799011

Designed by: Jacob Mejlsted, Joen Haahr Jensen, David Faurdal   Group: iGEM18_DTU-Denmark   (2018-10-07)


pCaMV - AmilCP - tCaMV

This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Contribution:

The DTU Biobuilders 2018 demonstrated successful integration of the construct into the genome of Aspergillus oryzae by PCR. Transformants exhibit a changed phenotype, with spore coloration altered from a light green to a dark green, thus indicating changes due to expression of the construct. Furthermore, this shows that PCaMV seemingly works in filamentous fungi.


[edit]
Categories
//function/reporter/color
Parameters
None