Generator

Part:BBa_K2796060:Design

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-08)


Vesicle attacker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 482
    Illegal BglII site found at 2067
    Illegal BamHI site found at 5120
    Illegal XhoI site found at 5
    Illegal XhoI site found at 6430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4685
    Illegal NgoMIV site found at 5705
    Illegal NgoMIV site found at 7006
    Illegal NgoMIV site found at 8117
    Illegal AgeI site found at 2947
    Illegal AgeI site found at 3567
    Illegal AgeI site found at 5100
    Illegal AgeI site found at 5988
    Illegal AgeI site found at 7490
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5154
    Illegal BsaI.rc site found at 6399
    Illegal SapI site found at 1657


Design Notes

We have to follow RFC 10 rules and consider its compatibility.


Source

NCBI

References