Generator

Part:BBa_K2796058:Design

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-07)


ECOB-(hsa-miR-135b-3p)-WPRE-SV40 poly(A) signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 807
    Illegal AgeI site found at 202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256


Design Notes

We need to think about following the RFC principles.


Source

NCBI

References