DNA

Part:BBa_K2796047:Design

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-04)


pGAL1- hsa-miR-769-5p

    We have synthesized miR-769-5p and constructed pSB1C3 vectors The result of agarose gel electrophoresis for miR-135b-3p, miR-942b-5p and miR-769-5p is shown below.
    2018 LZU-CHINA 29experimental Gel.png


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal XhoI site found at 512
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 70
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI.rc site found at 481


    Design Notes

    no


    Source

    NCBI

    ===References===