Coding

Part:BBa_K2796041:Design

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-04)


BirA-VP16

    The plasmid we design for device is shown below:
    2018 LZU-CHINA 47design1.png

    Agarose gel electrophoresis

  • The function of biotin-inducing system was tested by HEK 293T. Firstly, we stably transfected the vector that expressed birA-VP16 gene driven by pEF1a promoter into HEK 293T cells. And then, the plasmid with the ObirA-miniCMV-mRFP element is then transferred to the engineering 293T cells.
  • The result of agarose gel electrophoresis for BirA-VP16 is shown below.
    2018 LZU-CHINA 41experimental Gel.png


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]

    Design Notes

    no


    Source

    NCBI

    ===References===