Composite

Part:BBa_K2789025:Design

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)


Plac+RBS+lectin-OmpA+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 388
    Illegal AgeI site found at 577
    Illegal AgeI site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This a a standard expression pathway of K2789024.

We synthesized it in Pet28a plasmid and used PCR to add a prefix and a suffix. Then we used PstI and EcoRI to cut it and used ligase to link it to PsB1C3 backbone.

Primer: Forward:5‘-CGGAATTCGCGGCCGCTTCTAGAG AATTGTGAGCGGATAACAATTGAC-3’
FimH Reverse: 5’- GGTACTAGTAGCGGCCGCTGCAG CTCTAGTATATAAACGCAGAAAGGC-3’


Source

Lectin cds is originally from Green Algae.

References