Coding

Part:BBa_K2789021:Design

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)


PPN(endopolyphosphotase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1965
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Start with ATG and end with TAA, this is a standard coding sequence and it worked well in our project.You can use this part to build more complicated pathway!

Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-GCGGCCGC T TCTAG ATGGTTGTTGTTGGTAAATCTG-3'
rev: 5'-GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TGCGGCCGCTTAGTCG-3'


Source

This part comes from the genome of Saccharomyces cerevisiae and we carry out codon optimization for sequences to make it express best in E.coli.BL21.

References