Composite

Part:BBa_K2788010:Design

Designed by: Lindong He   Group: iGEM18_SZU-China   (2018-10-05)


PgpdA-HsbA-TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 903
    Illegal NheI site found at 2993
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BamHI site found at 3059
    Illegal XhoI site found at 693
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2553
    Illegal NgoMIV site found at 3695
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal BsaI.rc site found at 3409
    Illegal SapI site found at 315


Design Notes

In order to facilitate the replacement of the gene of interest, we added HindIII and KpnI restriction sites on both sides of the gene of interest.


Source

PgpdA comes from BBa_K2040101 HsbA comes from BBa_K2788000 TtrpC comes from BBa_K2040102

References

[1]Xiao G, Ying S H, Peng Z, et al. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J]. Scientific Reports, 2012, 2(483):483.

[2]Ye Zhang, Zhongren Lei, Haihong Wang, Jiqing Zhan. Prokaryotic expression and immunolocalization Beauveria bassiana HsbA protein [J] Chinese Agricultural Sciences, 2013,46 (21): 4534-4541.