Composite
Part:BBa_K2787014:Design
Designed by: Haoer Shi Group: iGEM18_ShanghaiTech (2018-10-09)
GFP under C9 orthogonal RBS with orthogonal 16S rRNA BBa_K2787042
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 798
Illegal NheI site found at 821 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1661
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1675
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707
Design Notes
Two parts were cloned into one plasmid to avoid issues brought by co-transformation.
Source
The part is constructed by restriction cloning BBa_K2787008 and BBa_K2787002 together into pSB1C3 backbone.