Composite

Part:BBa_K2787014

Designed by: Haoer Shi   Group: iGEM18_ShanghaiTech   (2018-10-09)


GFP under C9 orthogonal RBS with orthogonal 16S rRNA BBa_K2787042

The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSC9-GFP(BBa_K2787002) are linked into one plasmid to quantify the orthogonality of the TJU and C9 ribosome system.


Usage and Biology

A plasmid under control of C9 orthogonal RBS is constructed with the TJU orthogonal ribosome.

Shanghaitech2018-oribo-orbs-07-07.png
A2-B8-C9-Orthogonality.png

Expression of the target gene under the control of different orthogonal RBS with different orthogonal ribosomes are tested.

ShanghaiTech-TJU 16S.png

Expression of target gene under control of different RBSs with TJU orthogonal ribosome is tested, showing that the TJU ribosome is orthometric to other RBSs.

ShanghaiTech2018-c9 rbs.png

Expression of the target gene under control of the C9 orthogonal RBS with different orthogonal ribosomes to test the orthogonality of the C9 orthogonal RBS with other ribosomes. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 798
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


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