Coding
Part:BBa_K2787004:Design
Designed by: Weiyi Tang Group: iGEM18_ShanghaiTech (2018-10-08)
orthogonal ribosome 16s rRNA A2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 826
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Though most of the open database will show the 16s RNA only contains 1542bp, it is still necessary to obtain about 300 bp more since it will help to form the 16S rRNA precursor, otherwise, it may not function appropriately.
Source
The part is cloned from the Escherichia coli MG1655 genome and is mutated in Shine-Dalgarno sequence and 722 to 723 bp which will form a bulge proximal to the minor groove of the SD helix formed between the ribosome and mRNA. The orthogonal 16S rRNA sequence was published by Rackham, Oliver and Chin, Jason W. in their Nature Chemical Biology paper A network of orthogonal ribosome·mRNA pairs(doi:10.1038/nchembio719).