Composite

Part:BBa_K2787001

Designed by: Haoer Shi   Group: iGEM18_ShanghaiTech   (2018-10-09)


Constitutive promoter J23100 + orthogonal RBS B8 + GFP

This is a part used for justification and measurement of the orthogonality of the ribosomal 16sRNA and its corresponding ribosome binding site. It is composed of a constant expressing promoter(BBa_J23100), followed by a mutated ribosome binding site that can be specifically recognized by the orthogonal 16s rRNA B8 and a reporter protein GFP(BBa_E0040). The GFP would generate fluorescence only upon the presence of o-16SB8 while showing high orthogonality toward the innate 16s rRNA of E. coli.

Usage and Biology

Shanghaitech-o-GFP-09.png

a plasmid with GFP under control of B8 orthogonal RBS is constructed to test the orthogonality of B8 RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


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Categories
Parameters
None