Reporter

Part:BBa_K2782004:Design

Designed by: Dan Zhou   Group: iGEM18_BUCT-China   (2018-08-15)


This part can express red fluorescent protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The base sequence is edited based on the degeneracy of the protein, and the primary structure of the protein is not changed as much as possible. The amino acid at the active site of the protein cannot be altered.


Source

Synthetic construct clone pEY-sRFP-A, complete sequence Sequence ID: KX682231.1

References