Part:BBa_K2782002
This is an operon that uses fatty acids as signaling molecules.
Biodiesel refers to a fatty acid methyl ester or ethyl ester formed by the conversion of vegetable oil, animal oil, waste oil or microbial oil with methanol or ethanol. Biodiesel is a typical "green energy" with good environmental performance, good engine start-up performance, good fuel performance, wide range of raw materials, and renewable characteristics. Vigorously developing biodiesel has important strategic significance for sustainable economic development, promoting energy substitution, reducing environmental pressure, and controlling urban air pollution. We are proposing a newly designed fatty acid operon that acts as a molecular switch that signals fatty acids to regulate the expression of subsequent genes. It can act as a molecular switch with fatty acids as a signal to regulate the expression of subsequent genes. In combination with a repressor protein, RNA polymerase cannot bind to the promoter sequence, or RNA polymerase cannot move forward along the DNA, repress transcription, and then the gene cannot be expressed, and an appropriate amount of fatty acid is added to block the binding of the protein to the fatty acid. The gene can be expressed after exfoliation, and the more fatty acids, the more repressor proteins can be bound, and the better the gene expression. Using the fatty acid operon, we can design bacteria to add bacteria such as fluorescent proteins to the fatty acid operon. The amount of fluorescent protein can reflect the fatty acid content of the oil, so as to determine the acidity of the oil. It can be used as an indicator to judge the freshness of fat. Similarly, when it is used in biodiesel, it can be used to judge the progress of methyl esterification of biodiesel raw materials into biodiesel. This makes sense for promoting the development of biodiesel.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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