Protein_Domain

Part:BBa_K2772000

Designed by: Yan Xie   Group: iGEM18_DLUT_China_B   (2018-10-08)


Nanobody with aldehyde-based precursor label, can be converted to fixed-point aldehyde-modified Nano

It is anti-beta2 microglobulin nanobody with aldehyde-based precursor label,and it can be converted to fixed-point aldehyde-modified Nanobody by formylglycine-generating enzyme (FGE).It contains two sections：nanobody and ald tag,which is precursor of aldehyde label.Firstly it can cambein with beta2 microglobulin because of antigen-antibody interaction.Then its special lable allow fixed point fixing or other fixed point decoration possible.When you use it in your project,you can fix it on carrier with amino,acylhydrazine or oxyamino group without any purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 82
    Illegal BamHI site found at 444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 147


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Categories
Parameters
None