Intermediate

Part:BBa_K2771020:Design

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-06)


Ratiometric promoter characterization reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The choice of YFP and CFP is due to the separation of their excitation/emission spectrums. The use of an ECFP protein with a degradation tag is for the more accurate measurement of the promoter conditions at the time of sampling.


Source

Idea taken from Rudge et al. (https://doi.org/10.1021/acssynbio.5b00116), although executed with parts that were available in the Parts Registry.

References