Generator

Part:BBa_K2770007

Designed by: Jonathan Achter, Jamina Gerhardus, Christian Hake, Yannik Kaeseberg, Victor Macarron Palacios, Theresa Woermann   Group: iGEM18_TU_Darmstadt   (2018-09-30)


ICL1 Generator for S. cerevisiae

ICL1 codes for isocitrate lyase 1, natively occurring in the S. cerevisiae strain S288C [1]. The encoded enzyme catalyzes the key reaction of the glyoxylate cycle by transforming isocitrate into glyoxylate and succinate [2]. The molecular weight of the enzyme is 62.4 kDa[3].

Figure 1: Structure of the isocitrate lyase 1 (ICL1) with a molecular weight of 62.4 kDa.




The sequence of ICL1 was synthesized by IDT. We inserted the gene into BioBrick vector pSB1C3 vector and verified the DNA sequence by sanger sequencing (Eurofins Genomics).

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Figure 2: Figure 2: pSB1C3 plasmid including Icl1.



To learn more about ICL1 and its part in our project, please visit our [http://2018.igem.org/Team:TU_Darmstadt/Project/Glycolic_acid/S_cerevisiae wiki]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1302
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1630
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ ICL1 isocitrate lyase 1 [ Saccharomyces cerevisiae S288C] [1].
  2. ↑ > ICL1 Saccharomyces cerevisiae [2]
  3. ↑ > ICL1 / YER065C Overview [3]
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Categories
Parameters
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