Part:BBa_K2765053:Design

Complete roGFP2-Orp1 expression cassette started by the promoter FBA1t.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1480
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1792
  

Design Notes

NO

Source

Connected pFBA1 and roGFP2-Orp1 by OE-PCR. And ligated the fragment to the plasmid pESC, and pFBA1-roGFP2-Orp1-CYC1t was obtained by PCR.