Composite

Part:BBa_K2765050:Experience

Designed by: Fa He   Group: iGEM18_BIT-China   (2018-10-08)


We ligated the promoter to roGFP2-Orp1 by Overlap Extension PCR and ligated it to the plasmid pESC-Trp containing the terminator cyc1 by enzyme digestion.

T--BIT-China--iGEM2018-PartsOutput-TEF2-protein.png

After sequencing verification, we transferred the correct plasmid into yeast for expression. Then we measured the fluorescence intensity of roGFP2-Orp1(Excitation wavelength is 488nm and the emission is followed at 515nm), which improved that the part can work properly.

T--BIT-China--iGEM2018-PartsOutput-TEF2experiment.png

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