Coding
Part:BBa_K2764000:Design
Designed by: CHU Ora-In Group: iGEM18_Hong_Kong_HKUST (2018-10-03)
Alkane channel protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 651
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 303
Illegal NgoMIV site found at 516 - 1000COMPATIBLE WITH RFC[1000]
The part sequence has been codon-optimized for E. coli.
Design Notes
Added promoter and terminator for expression
Source
outer membrane protein AlkL from Pseudomonas oleovorans