Composite

Part:BBa_K2762006:Design

Designed by: Ting-Shou Liu   Group: iGEM18_NCKU_Tainan   (2018-09-29)


PT7-B0034-rbcX-B0034-rbcS-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first codon optimized the rbcL sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with HindIII and SpeI.


Source

Codon oprimized Synechococcus elongatus PCC7002.

References