Coding

Part:BBa_K2762000:Design

Designed by: HUI YEE NG   Group: iGEM18_NCKU_Tainan   (2018-09-28)


rbcL (Large subunit of the ribulose-bisphosphate carboxylase/oxygenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
    Illegal AgeI site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first codon optimized the rbcL sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with HindIII and SpeI.

Source

Codon oprimized Synechococcus elongatus PCC7002.

References