Composite

Part:BBa_K2761010:Design

Designed by: Alan Eduardo Ávila Ramírez   Group: iGEM18_Tec-Monterrey   (2018-10-10)


Inducible message generator (RT/msr-msd)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 435
    Illegal BamHI site found at 1355
    Illegal BamHI site found at 1419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used with E. coli BL21 D3 only.

Source

Based on SCRIBE research.

References

Farzadfard, F., & Lu, T. K. (2014). Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science, 346(6211), 1256272.

Shipman, S. L., Nivala, J., Macklis, J. D., & Church, G. M. (2017). CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria. Nature, 547(7663), 345.