Composite

Part:BBa_K2760030:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-12)


SP1-Nsp4 characterization construct L. rhamnosus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 202
    Illegal AgeI site found at 784
    Illegal AgeI site found at 896
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Optimized for L. rhamnosus


Source

This plasmid expression vector has a spaCBA promoter, it contains a specific RBS for L. rhamnosus GG, the signal peptide SP1-Nsp4, M-cherry (BBa_J18932) as the fluorescent reporter with a His-Tag (6 histidines) attached to it; and the terminator 667.



References

[1] Han, S., Machhi, S., Berge, M., Xi, G., Linke, T., & Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli. AMB Express, 7, 93. http://doi.org/10.1186/s13568-017-0394-1