Composite

Part:BBa_K2760022:Experience

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-10)


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Applications of BBa_K2760022

The aplication of this signal peptide is not only to serve as a positive control for characterization of secretion signal peptides but for secretion of recombinant proteins and characterization by fluorescence. This particular construct was created to characterize the efficiency of the signal peptide DsbAss.

The team iGEM18 Tec GDL managed to run western blots and electrophoresis as a way to characterize this construct. (http://2018.igem.org/Team:TecMonterrey_GDL/GeneticE). Since it has a His-Tag, it was detected on the Western Blot assays.


The following image contains our Western blot results. http://2018.igem.org/File:T--TecMonterrey_GDL--GenericE--8.png

<img src="http://2018.igem.org/File:T--TecMonterrey_GDL--GenericE--8.png">

User Reviews

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