Part:BBa_K2759012
GS Linker HisTag GS Linker PBP
We present the certain part of the penicilin binding protein 1b(PBP 1b) with HisTag and GS linker. PBP 1b is a protein on the transmembrane of the bacteria, which involves peptidoglycan synthesis. Certain B-lactam antibiotics interact with the PBP 1b by inhibiting the cell wall synthesis. This composite part contains GS linker which allows the conjugation with different proteins and HisTag to perform Western Blot experiment.
Usage and Biology
Penicillin Binding Protein 1b is major enzyme for peptidoglycan synthesis. Escherichia coli PBP1b is a bifunctional transglycosylase, also known as peptidoglycan glycosyltransferase or murein synthase. It is revealed that particular part of the PBP 1b protein interacts with B-lactam antibiotics.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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