Composite

Part:BBa_K2759012

Designed by: Azra Atabay   Group: iGEM18_Bilkent-UNAMBG   (2018-10-09)


GS Linker HisTag GS Linker PBP


We present the certain part of the penicilin binding protein 1b(PBP 1b) with HisTag and GS linker. PBP 1b is a protein on the transmembrane of the bacteria, which involves peptidoglycan synthesis. Certain B-lactam antibiotics interact with the PBP 1b by inhibiting the cell wall synthesis. This composite part contains GS linker which allows the conjugation with different proteins and HisTag to perform Western Blot experiment.

Usage and Biology

Penicillin Binding Protein 1b is major enzyme for peptidoglycan synthesis. Escherichia coli PBP1b is a bifunctional transglycosylase, also known as peptidoglycan glycosyltransferase or murein synthase. It is revealed that particular part of the PBP 1b protein interacts with B-lactam antibiotics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None