Conjugation

Part:BBa_K2759003:Experience

Designed by: Mehmet Ali Hoşkan   Group: iGEM18_Bilkent-UNAMBG   (2018-10-09)


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Applications of BBa_K2759003

T--Bilkent-UNAMBG--itasser.png

This is the 3D model of the translation of the conjugated part (Bacterioferritin (BFR) with sfGFP conjugation) via iTASSER tool.

T--Bilkent-UNAMBG--003digest.png

Figure: Bba_K2759003 part is verified by digesting the part with NcoI and KpnI restriction enzymes

T--Bilkent-UNAMBG--sfgfpfor003.png

In order to test the functionality of the BBa K2759003 part it is cloned to be regulated under proD promoter. BFR sfGFP conjugation under proD promoter regulation construct is translated and sfGFP signal is visualized.

User Reviews

UNIQc3786207a949ee0d-partinfo-00000000-QINU UNIQc3786207a949ee0d-partinfo-00000001-QINU