Regulatory

Part:BBa_K2753055

Designed by: WEI KUANGYI   Group: iGEM18_GreatBay_China   (2018-10-02)


UPJ23119

The promoter UP119 is a modified variant of J23119 consensus promoter (Part:BBa_J23119) by adding an up-element. This alteration makes UP119 stronger than the original J23119.


For comparing the strength of two J23119 variants with J23119, we cloned them with a sfGFP onto three plasmids of distinctive copy numbers.

  • pUC20: ~500
  • pR6K: ~15
  • pSC101: ~1


The strength of them are characterised by measuring the fluorescence using a Flow Cytometry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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