Composite

Part:BBa_K2751013:Design

Designed by: Ming-Sheng Hsu   Group: iGEM18_NYMU-Taipei   (2018-10-09)


pCMV-ALB-mEGFP internal control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 268


Design Notes

The ALB tagged mEGFP is under the control of a CMV promoter.

Source

mEGFP is mutation product of EGFP, which is amplified by conducting PCR on plasmids provided by Dr. Chung-Chih Lin of NYMU. ALB is amplified by PCR on HEK293 genome. CMV promoter is amplified by PCR on pCMV-Mycj-XFP from Dr. Nien-Jung Chen of NYMU.

References