Reporter

Part:BBa_K2751011:Design

Designed by: Ming-Sheng Hsu   Group: iGEM18_NYMU-Taipei   (2018-10-09)


mEGFP skel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 724
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did a point mutation at 206a.a. (Ala  Lys). This mutation changed the Van-Der-Waals force between identical EGFP molecules and decreased EGFP’s propensity to form dimers.


Source

EGFP is amplified by conducting PCR on plasmid obtained from Dr. Chung-Chih Lin of NYMU.

References