Coding

Part:BBa_K2740000:Design

Designed by: Zhaoqing Hu   Group: iGEM18_Nanjing-China   (2018-09-16)


Pnif-nifB-nifH-nifD-nifK-nifE-nifN-nifX-hesA-nifV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Length of the gene cluster is more than 10kb, which makes it hard to manipulate without mistakes, so the DNA sequencing seems necessary. And Adjustment to the gene copy number of individual subparts may improve the efficiency of nitrogen fixation.


Source

Paenibacillus polymyxa CR1

References